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Lux biosensors for antibiotic detection: The contribution...
来自 : www.onacademic.com/detail/jour
发布时间：2021-03-24
Lux biosensors for antibiotic detection: The contribution from reactive oxygen species to the bactericidal activity of antibiotics Author: Zavil’gel’skii, G. B. Kotova, V. Yu. Mironov, A. S.
Journal: Russian Journal of Physical Chemistry B
Issue Date: 2015
Abstract(summary):
Highly sensitive specific lux biosensors based on Escherichia coli bacteria have been designed for the detection of tetracycline, β-lactam, and quinolone antibiotics. The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the luxCDABE reporter genes of Photorhabdus luminescens is performed under the control of the inducible promoter genes tetA, ampC, and cda, respectively. The basic characteristics of the lux biosensors (sensitivity threshold, response amplitude, and response time) have been determined. The contribution from reactive oxygen species to the bactericidal activity of quinolone antibiotics has been evaluated.
Page: 454-460
The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the luxCDABE reporter genes of Photorhabdus...onAcademic ；Science；Technology；Medicine；researchHighly sensitive specific lux biosensors based on <em class="EmphasisTypeItalic ">Escherichia coli</em> bacteria have been designed for the detection of tetracycline, β-lactam, and quinolone antibiotics. The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the <em class="EmphasisTypeItalic ">lux</em>CDABE reporter genes of <em class="EmphasisTypeItalic ">Photorhabdus luminescens</em> is performed under the control of the inducible promoter genes <em class="EmphasisTypeItalic ">tetA</em>, <em class="EmphasisTypeItalic ">ampC</em>, and <em class="EmphasisTypeItalic ">cda</em>, respectively. The basic characteristics of the lux biosensors (sensitivity threshold, response amplitude, and response time) have been determined. The contribution from reactive oxygen species to the bactericidal activity of quinolone antibiotics has been evaluated.Lux biosensors for antibiotic detection: The contribution from reactive oxygen species to the bactericidal activity of antibiotics
Journal: Russian Journal of Physical Chemistry B
Issue Date: 2015
Abstract(summary):
Highly sensitive specific lux biosensors based on Escherichia coli bacteria have been designed for the detection of tetracycline, β-lactam, and quinolone antibiotics. The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the luxCDABE reporter genes of Photorhabdus luminescens is performed under the control of the inducible promoter genes tetA, ampC, and cda, respectively. The basic characteristics of the lux biosensors (sensitivity threshold, response amplitude, and response time) have been determined. The contribution from reactive oxygen species to the bactericidal activity of quinolone antibiotics has been evaluated.
Page: 454-460
The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the luxCDABE reporter genes of Photorhabdus...onAcademic ；Science；Technology；Medicine；researchHighly sensitive specific lux biosensors based on <em class="EmphasisTypeItalic ">Escherichia coli</em> bacteria have been designed for the detection of tetracycline, β-lactam, and quinolone antibiotics. The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the <em class="EmphasisTypeItalic ">lux</em>CDABE reporter genes of <em class="EmphasisTypeItalic ">Photorhabdus luminescens</em> is performed under the control of the inducible promoter genes <em class="EmphasisTypeItalic ">tetA</em>, <em class="EmphasisTypeItalic ">ampC</em>, and <em class="EmphasisTypeItalic ">cda</em>, respectively. The basic characteristics of the lux biosensors (sensitivity threshold, response amplitude, and response time) have been determined. The contribution from reactive oxygen species to the bactericidal activity of quinolone antibiotics has been evaluated.Lux biosensors for antibiotic detection: The contribution from reactive oxygen species to the bactericidal activity of antibiotics
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发布于 ： 2021-03-24
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